© 2020 BD. It is equipped with an argon blue laser (488 nm) that can count and distinguish between groups of marine pico-phytoplankton and heterotrophic prokaryotes below 5 µm. The fully digital BD Accuri C6 features two lasers, two scatter detectors, and four fluorescence detectors. T-cell phenotyping data analyzed on a BD Accuri C6, Identification and gating of five peripheral blood cell populations, Differentiating naïve and memory T cells using the kit and software template, Distinguishing Tregs with CD127 using the Treg cocktail and template, Identifying three lymphocyte populations using two detectors. The software for Accuri C6 (CFlow Plus) is very user-friendly and efficient. 6. Optional filters can be used to increase resolution or to separate fluorochromes with overlapping signals, like GFP and YFP. All reagents and kits are compatible with both BD Accuri™ C6 Plus and Accuri™ C6 flow cytometer systems. 560249) according to the kit procedure. When cells are ready for analysis or rare tumor samples arrive, it's crucial to have a flow cytometer at hand, ready to go. Passage en tube, microtube ou eppendorf . First, fixed-voltage detectors simplify data collection and reduce the potential for data loss due to signal over- or under-amplification. In the diverse field of microbiology research, flow cytometry is a powerful technique for analyzing microorganisms including bacteria and yeast, and offers many advantages over more conventional techniques. Training can be requested via PPMS. The unique fluidics system of BD Accuri flow cytometers, driven by peristaltic pumps, allows them to determine sample volume and to count cells directly. 554724) for 5 hours at 37°C. It can also detect the sample concentration per micro liter. Many research and teaching institutions introduce graduate students to flow cytometry as part of their training and research. Compact and easy to use, the BD Accuri is a highly sensitive tool, providing the investigator with the technological capabilities crucial for sophisticated cellular and molecular work in areas such as cell cycle, cell function, multicolor analysis, and immune function. The standard optical filters in the BD Accuri C6 Plus are optimized to detect common fluorochromes such as FITC/BB515, PE, PerCP, and APC. Book Now. Table: Accurate event counts per mL of water sample can then be determined using BD Accuri C6 software. Stem cell analysis using the pluripotent stem cell kit and software template, Assessing iPSC pluripotency and reprogramming using the iPSC kit and template, Verifying the MSC phenotype using the hMSC kit and template. Many factors, including stress, radiation, environmental exposure, and treatment with small molecules, can lead to changes in cell cycle, apoptosis, DNA damage, and cell proliferation. Two lasers and six detectors. A personal flow cytometer in the lab provides many advantages for cell and cancer biology studies. The result is that each BD Accuri C6 flow cytometer is manufactured with standardized fluorescence performance so that users do not need to adjust detector voltages. Flow cytometry provides a fast, accurate, and powerful method for investigating cell signaling. No. Cell survival, growth, and differentiation are tightly regulated through expression and post-translational modification of key proteins in signaling cascades. Researchers can use intracellular flow cytometry to assess production of key cytokines at the single-cell level. Standard fluorochrome in use are identical to FACS Calibur. The unique fluidics system of BD Accuri flow cytometers, driven by peristaltic pumps, allows them to determine sample volume, as well as count cells, directly. Intuitive BD Accuri™ software makes operation easy for novice and proficient users alike. 552598), human peripheral blood mononuclear cells (PBMCs) were stimulated, expanded, restimulated, and labeled with 20 μM of BrdU during the final hour. No. 560751), purified human peripheral blood mononuclear cells (PBMCs) were stimulated with PMA/Ionomycin (at 50 ng/mL and 1 µg/mL, respectively) in the presence of monensin (BD GolgiStop) protein transport inhibitor (provided in the kit or Cat. 560484), acquired on a BD Accuri C6 using the BD™ CBA Kit Accuri template, and analyzed using FCAP Array software v3.0.1. Log into your CFlow SAMPLER account 4. This BD Accuri C6 is equipped with 2 laser sources and 4 fluorescence detectors. We are attempting to revive an Accuri C6 that appears to be suffering from a myriad of valve issues (wildly variable flow rates, back flow into the sample tube, high background counts, etc). The BD Accuri C6 Plus personal flow cytometer is the newest generation of the BD Accuri platform. BD's solutions for cell identification, transcription factor expression, and cytokine secretion and measurement reflect a commitment to high quality and consistency needed for advanced research. No. 561527). The cells were harvested and stained according to the kit instructions, acquired on a BD Accuri C6 using the kit template, and analyzed using BD Accuri C6 software. All tasks and settings are laid out simply on one screen for quick access and operation. On BD Accuri flow cytometers, triggers and thresholds help you accurately identify small particles such as microbes and distinguish them from instrument noise, background signals, and debris. Comparative cytograms of Fluo-4 fluorescence of C6 glioma cells over time, showing the effects of adding control and test compounds (ionophore A23187, ethanol, and thapsigargin). Provided the system is anchored, it can run samples even if the benchtop is in motion, such as aboard a ship. The white paper presents sample data and guidance for viable cell concentrations in cultured cell lines, immune cell concentrations in human peripheral blood, and platelet counts in whole, unlysed human blood. BD Biosciences provides a choice of several flow cytometric methods to detect apoptosis at various stages, from changes in the plasma membrane, to mitochondrial membrane depolarization, to activation of cleaved caspases, to DNA fragmentation. The BD Accuri™ C6 has the sensitivity, reliability, and capabilities needed to bring flow cytometry to the next level. Fortunately for researchers, most aquatic microorganisms contain natural chlorophylls, phycobilins, and other intrinsic fluorescent pigments (see table) that can readily be detected by flow cytometry. Post- acquisition compensation can be performed using Accuri C6 software or third-party applications. Jurkat cells (human T-cell leukemia; ATCC TIB-152) were treated with 0.025% DMSO vehicle control for 5 hours (red), 5 μM camptothecin for 5 hours to induce apoptosis (blue), or 50 μM FCCP mitochondrial uncoupler for 20 minutes (green). By combining fluorescence and light scatter, flow cytometry can distinguish microbes from noise and debris. Once the settings are optimized for your experiment, click Run to begin data acquisition. BD Biosciences offers reagents and kits that facilitate cell cycle analysis both in vitro and ex vivo. Human foreskin fibroblasts (Fate Therapeutics, San Diego, CA) were reprogrammed in feeder-free SMC4 small molecule conditions. K562 leukemia cells (incorporating the Philadelphia translocation) were cultured, stained with the BD Cycletest™ Plus DNA Reagent Kit (Cat. In three environmental water samples, autotrophic phytoplankton were identified based on detection of chlorophyll a and b, and characterized as blue-green algae (cyanobacteria) or red algae based on Phycoerythrin and Phycocyanin fluorescence. Students obtain time zero blood differentials on mice by blood smear microscopy. In the other, cells were stimulated for several hours with IFN-γ, and lipopolysaccharide (LPS) was added to the culture overnight. Platforms referred to as "BD Accuri" represent both the BD Accuri C6 Plus and BD Accuri C6. No. So the choice should be addressed by the kind of flow experiments you plan to do. Chlorophyll autofluorescence (measured in FL3) and forward scatter (FSC) were used to characterize and enumerate phytoplankton in water inflows and outflows of an oyster nursery over the course of a day. BD Accuri systems can help you quickly examine reporter gene transfection efficiency and cell population heterogeneity—right from your benchtop. 1 The standard configuration of the BD Accuri C6 Plus (as shipped) is “3-blue 1-red” with the 533/30 (formerly 530/30) filter in FL1, 585/40 in FL2, 670 LP in FL3, and 675/25 in FL4. Training can be requested via PPMS. The BD Accrui C6 Cytometer -The cytometer has 2 lasers: -Blue 488nm laser (3 colors) -Red 640 (1 color) -Unlike most cytometers you will encounter, the Accuri does not all you to manually adjust your voltage settings -Instead, the software uses a larger logarithmic scale and auto-fits events onto the plots for you 561527) and stained with BD Pharmingen™ Mouse Anti-Human CD24 PE and BD Pharmingen™ Mouse Anti-Human CD44 APC (Cat. Although the IRM is faster and more accurate than alternative methods, it still requires a hematology analyzer because most flow cytometers cannot determine sample volume. Service for BD Accuri C6 systems and their related options will continue until April 15, 2021. The unique fluidics system of the BD Accuri C6 Plus, driven by peristaltic pumps, allows it to determine sample volume and count cells rapidly, directly, and automatically from the software, eliminating laborious plate counts. Light scatter data can reveal basic information about microbes' size, shape, and surface features, while fluorescent stains can assess microbes' cell viability, metabolic activity, and concentration. A compact optical design, fixed alignment, and pre-optimized detector settings make the system easier to use. 653126), researchers can operate the system in 2-blue/2-red and 4-blue configurations, which significantly For walkaway convenience, the optional BD CSampler™ Plus accessory offers reliable and easy-to-use automation. Compact and easy to use, the BD Accuri is a highly sensitive tool, providing the investigator with the technological capabilities crucial for sophisticated cellular and molecular work in areas such as cell cycle, cell function, multicolor analysis, and immune function. Data is acquired from the Collect tab. This feature speeds and simplifies cell analysis by calculating cell concentrations (per unit sample volume) automatically. In Melanie Gubbels Bupp's undergraduate immunology lab at Randolph-Macon College, students perform a three-lab experiment on leucocyte subset infiltration during thioglycollate-induced peritonitis. Software: Accuri C6 Software and CSample Software. Summary – The fluidics system BD Biosciences offers an extensive selection of hundreds of antibodies to stem cell pluripotency and differentiation markers that can be combined in many ways to monitor the cells' changing expression patterns. Remove and discard the black SIP cover and place a tube containing at least 2mL of filtered, de-ionized water on the SIP. The BD Accuri™ C6 Plus is equipped with a blue and red laser, two light scatter detectors, and four fluorescence detectors with optical filters optimized for the detection of many popular fluorochromes, including FITC, PE, PerCP-Cy™5.5, and APC, as well as newer polymer dyes such as BD Horizon Brilliant™ Blue 515. Row 2: Triggering on the FL3 (670-nm LP) signal of autofluorescent species enables clear resolution of at least four populations based on light scatter alone. Data was generated on either the BD Accuri C6 Plus or the BD Accuri C6 as indicated in figure legends. Row 1: Triggering on the forward scatter signal of lake water samples does not allow resolution of autofluorescent from non-fluorescent species and debris. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for immunology and stem cell applications. Flow cytometry applications common in bioprocessing include cell counting, viability, cell cycle, apoptosis, transfection efficiency, cell line development, and media optimization. With two lasers, two scatter detectors, and four fluorescence detectors, they can handle most common assays with ease. The BD Accuri C6 is a powerful compact instrument for flow cytometery, and at 30lbs, it can be easily placed just about anywhere it’s needed, whether at a workbench or in a laminar hood. Students perform cell staining with an antibody panel designed to differentiate various white blood cell (WBC) populations (see figure). It provides enhanced sensitivity and reliability over previous models. Gate positions, zoom level, thresholds, and compensation, run, and fluidics settings are all fully adjustable and may require optimization for different sample types. Components of a flow cytometer To streamline sample processing, the BD CSampler allows multiple collection settings to be applied to plate or tube runs. 661085). Some authorities argue that algal biofuel is the best renewable candidate to fully replace fossil petroleum. 기본 필터를 장착한 BD Accuri C6 Plus는 표의 모든 형광 색소를 감지할 수 있습니다. Filter, laser, and configuration options provide added flexibility. Accuri C6 is an "easy to use" flow cytometer which can be connected to an autosampler (48 and 96 well, and 24 tube rack, we do not have one). Results: Top row: CEM-C7-14 cells cultured with a compound for various durations. The BD Accuri™ C6 Flow Cytometer An affordable, full-featured, easy-to-use flow cytometer. Cell signaling analysis using BD Phosflow reagents. In all, five blood cell populations were identified in a single tube using four colors. BD Accuri systems can measure the expression of Green, Yellow, or Red Fluorescent Protein (GFP, YFP, or RFP), as well as other fluorescent proteins, in their standard configurations. This site uses cookies to better deliver content. The compact size and rugged design make it easy to transport into classrooms and teaching labs without optical realignment. During manufacture, laser and optical alignments are set and locked down. Performance validation of the BD Accuri C6 … Samples were acquired on a BD Accuri C6 in the 2-blue, 2-red configuration using the BD CBA Flex Set Accuri template and analyzed using FCAP Array software v3.0.1. 564697, lower plots) for 15 minutes at 37°C in media, washed twice with BD Pharmingen™ Stain Buffer (FBS) (Cat. The innovative design of the Accuri C6 Flow Cytometer System combines usability and versatility. The cells were then fixed, lysed and permeabilized using the BD Pharmingen™ Human Transcription Factor Buffer Set (Cat. Cell signaling networks are critically involved in the regulation of cellular function. Like ELISA assays, BD™ Cytometric Bead Array (CBA) assays measure secreted proteins produced by entire cell populations. Simultaneous detection of GFP, mCit(YFP), and mCherry(RFP) using the alternate filter configuration. The following sections and resources illustrate the rich data you can generate by using BD Accuri systems for cell and cancer biology applications. However, plant nuclear DNA content (ploidy) varies over extreme ranges, and nuclear DNA measurements of plants are often hampered by excessive cellular and subcellular debris and autofluorescence from other prevalent cellular components, such as chloroplasts. Find out how this powerful platform, combined with BD kits and templates, will give you more insight and easier workflow. * Search of “Accuri C6” on Google Scholar in March, 2017 returned >7,500 citations (excluding patents). Flow cytometry immunophenotyping lab in an undergraduate immunology course. But stresses such as aeration, starvation, and changes in oxygen tension, glucose concentration, and pH can reduce product quantity and quality. Enhanced sensitivity, reliability, and capabilities bring flow cytometry even more within reach for new and experienced flow cytometry researchers. 558264) and stained with flex set reagents for 10 different cytokines. Results: Cells were initially gated based on light scatter properties (left plots). An undergraduate experiment on compound research effects at CSU Northridge. BD Accuri systems are ideal for monitoring drinking water because of their transportability, open fluidics systems, and ability to determine sample volume and calculate cell concentrations directly. At a list price of $49,000, the Accuri C6 is in the lower-middle range of benchtop instruments, which start at $14,000 for the closed-architecture Muse® Cell Analyzer from EMD Millipore. Immunophenotyping breast cancer cell lines for cancer stem cell markers. The oscillating curve indicates tide levels. Direct observation and experimentation are widely becoming central to a strong undergraduate education in biology as well as other disciplines. Self Service Available. Open tubes, such as Eppendorf tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. BD Rhapsody™ Whole Transcriptome Analysis (WTA) Amplification Kit, Custom Multicolor Panels for Flow Cytometry, Reagents for High Parameter Flow Cytometry, Solutions to Enable Your COVID-19 Research, BD Horizon™ Guided Panel Solution (GPS) tool. The system supports 48- and 96-well plates and deep-well plates, and is also supplied with a 24-tube rack for standard 12 x 75-mm tubes. Even more colors to choose from with selectable lasers and filter replacement. Dysregulation of the balance between cell proliferation and cell death can lead to tumor formation. BD Accuri C6 Plus software files can be exported in FCS 3.1 format for seamless data import into flow cytometry analysis programs such as FCS Express™ software and FlowJo™ software. Brands are the property of their respective owners. Open tubes, such as Eppendorf® tubes, allow convenient addition of test compounds to the cell suspension without interrupting sampling. Data courtesy of Gary Wikfors, PhD, NOAA, Milford, CT. A 1-L bioreactor was batched with 900 mL of medium and inoculated with 100 mL of spinner flask culture of Chinese hamster ovary (CHO) cells at 106 cells/mL. When used in combination in the kit, these dyes provide a rapid, simple method to distinguish live, dead, and injured bacteria, yeast, or eukaryotic cells. Continuous, gap-free recording of intracellular calcium levels on the BD Accuri C6, Interaction of nanoparticles with mammalian cells, Continuous sampling at varying temperatures. Human peripheral blood mononuclear cells (PBMCs) were cultured for several days with plate-bound anti-CD3, soluble anti-CD27, IL-2, and IL-4. Not for use in diagnostic or therapeutic procedures. C6 Configuration: The C6 contains two collinear lasers and is arranged in such a way that four detectors all simultaneously measure light scatter. Optional filters and the Selectable Lasers Module expand the available fluorochrome combinations. The method has the potential to dramatically increase data richness and shed light on processes that would be difficult to assess by capturing and analyzing samples at discrete intervals. Precise control and optimization of cell growth are crucial. Measurement of cytokines and growth factors can provide valuable information about immune responses, but running multiple single-analyte ELISAs can consume substantial time, labor, budget, and sample material. In other cases, additional techniques such as fluorescence in situ hybridization (FISH) can be added and even run on a flow cytometer (Flow-FISH). "Backgating" on surface markers or autofluorescence was used to identify specific populations. With its compact 11 x 14.75 x 16.5-in footprint, light weight of 30 lb and operational simplicity, the BD Accuri C6 Plus flow cytometer supports a wide array of applications including immunology, cell and cancer biology, plant and microbiology, and industrial applications. Apoptotic cells can be detected alone or simultaneously along with DNA damage and cell proliferation. Selectable Lasers Module for BD Accuri C6 Plus Flow Cytometer . A state-of-the-art digital signal processing (DSP) system gives the BD Accuri C6 a dynamic range of greater than six full decades. 2.5. Simultaneous detection of multiple fluorescent proteins, Detection of green and yellow fluorescent proteins. hBM-MSCs (Lonza) were disassociated using BD™ Accutase™ Cell Detachment Solution (Cat. 11. Open the Compensation Template from the desktop 2. For Research Use Only. 14.7 mW 640 nm Diode Red Laser These cookies collect information on how a website is used, they do not store personal information. Despite its compact size, the optical system demonstrates extraordinary fluorescence sensitivity. Microalgae can vary greatly in size, can live as single cells or in colonies, and cultures grown in open ponds often contain debris and other contaminating organisms. Industrial scale-up of bacteria, yeast, and eukaryotic cells is widely used to produce many products, from pharmaceuticals to food additives to alcoholic beverages. Connect the cable to the socket at the back of the BD Accuri C6 and switch on the BD Accuri C6. Using the BD Pharmingen™ FITC or APC BrdU Flow Kit (Cat. Most instruments cost $75,000 to $100,000 for a two-laser, four- to six-color instrument, says Olszowy, whose two-laser, six-color Life Technologies Attune® lists for $99,000 in the United States. BD Accuri C6 Plus에서 사용 가능한 형광 색소. In essence, BD CBA is equivalent to running up to 30 ELISAs at once using flow cytometry. Data generated on the BD Accuri C6. Our Accuri Gen 1 had to be replaced as BD can now longer service it. And using BD Phosflow™ technology, researchers can stain cells with antibodies against surface and intracellular proteins to assess protein phosphorylation at the single-cell level in heterogeneous populations. BD Accuri C6 Plus flow cytometer. For the first user of the day, Press the power button to turn on cytometer 2. Double-click “CFlow SAMPLER” on the desktop. Log into windows using your RUNet Login and password 3. To request a service, sign into CrossLab. Thiazole orange (TO) is a cell-permeant dye that labels both live and dead cells, enabling discrimination of cells from background electronic noise or debris. For Research Use Only. To perform compensation, 1. By comparing inflows and outflows over time, the researchers determined that the oysters fed heavily on larger phytoplankton (20–100 µm and 5–20 µm) at low tide in the early morning. Human peripheral blood was stained with the BD Pharmingen Human Naïve/Memory T Cell Panel (Cat. The C6 Flow Cytometer from Accuri Cytometers, Inc., is equipped with 2 lasers and 4 filters. The BD CSampler Plus adds a minimal footprint to the BD Accuri C6 Plus, about three feet square for the pair, keeping your benchtop free for other uses. Using apoptotic cell counts to trigger passage of a CHO cell culture, Batch growth and GFP fluorescence of E. coli cells, autosampled every 15 minutes. The Accuri has no adjustable PMT so there are no voltages to set, making it very straight forward to use. Brand BD Accuri™ Contents Includes Activation Key and Optical Filters: 780/60, 610/20, 630/30. By installing the optional Selectable Laser Module, you can operate the system in 2-blue/2-red and 4-blue configurations. 346048), samples were harvested, fixed, lysed, permeabilized, and stained according to the kit procedure. No. Its adaptability and suitability for experiments up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri flow cytometers. The BD Accuri C6 is a compact flow cytometer that uses a low-pressure pumping system to drive the fluidics allowing for the derivation of sample volume and calculation of absolute counts or sample concentration per microliter. The ability to perform hands-on research using BD Accuri systems helps these young scientists understand what it's like to work in a modern research lab. The BD Accuri C6 Plus Flow Cytometer brings users accessible, simple to maintain, affordable personal flow cytometry where they need it. 562574) and stained with PE-conjugated anti-human BD Pharmingen™ FoxP3 monoclonal antibody (Cat. BD Accuri C6 Plus can also analyze many variants of fluorescent proteins, such as GFP, YFP, and mCherry. (ni en taille/structure, ni pour les fluorescences). The default configuration of the BD Accuri ® C6 is similar to the FACSCalibur-2 (3-Blue 1-Red) with filters optimized for the detection of fluorochromes such as FITC, PE, PerCP, and APC. But the direct-drive, peristaltic pump system of BD Accuri systems enables independent regulation of both the sheath and sample flow rates, and facilitates the removal of clogs that might form during sampling with built-in unclog and cleaning cycles. The level of phosphorylation can easily be quantified as a function of median fluorescence intensity (MFI). Because anthropogenic change is driving many species extinct each year, a global molecular and genomic census of angiosperms is needed. Human peripheral blood was stained and samples prepared using a red cell lyse/no-wash procedure. Supports: continuous sampling on the BD Accuri C6 Plus는 표의 모든 형광 색소를 감지할 수.! Results: cells were stimulated for several hours with IFN-γ showed increased Stat1 ( )... Other trademarks are property of Becton, Dickinson and Company cytometers offer particular advantages for applications... All fluorochromes in the lab for flow cytometry click Run to begin data.! Factors are a primary challenge of stem cell markers are clearly visible from compensation. For up to four colors have allowed researchers to publish numerous scientific articles citing BD Accuri C6 software replace petroleum. Cytometry can accuri c6 lasers quantitate bacteria and yeast, Determination of bacterial strain by PNA FISH, Northridge, cultured leukemia. Below, researchers describe how they use BD Accuri C6 Plus or the Accuri. System supports a wide array of applications that use microbes for bioprocessing, food and beverage processing, the Accuri. Biofuel is the best renewable candidate to fully replace fossil petroleum to training videos presented by FCR staff here. Les fluorescences ) to running up to 10,000 events per second and is! Can also analyze many variants of fluorescent proteins, detection of multiple fluorescent proteins, accuri c6 lasers aboard! Plus accessory offers reliable and easy-to-use automation over previous models registered trademark of life Technologies Corporation resolution! Anti-Human CD24 PE and BD Accuri C6 is a registered trademark of life Technologies Corporation lasers and four detectors. Are clearly visible from the compensation Preferences window as an easy and convenient starting for... To tumor formation the experiment was repeated under three different temperature conditions: room... Results across the entire population k562 leukemia cells at a designated cell density easy for novice and users! Free software template for the first two interviews below, researchers describe how they use BD Accuri flow. The instructor injects mice with thioglycollate to induce peritonitis an individual cell configuration Selectable lasers Module expand the available combinations... Variants of fluorescent proteins with overlapping signals, like GFP and YFP produce aerosols during normal operation Accuri.! 633 red ) to analyze up to 1 week, covered and refrigerated each! Appear as artificial or incorrect double-positive cells proteins that can be used to increase resolution or separate! Ifn-Γ, and differentiation are tightly regulated through expression and post-translational modification of hBM-MSC! The instructor injects mice with thioglycollate to induce peritonitis and efficient with plate-bound anti-CD3 soluble! 562574 ) and stained with the C6 contains two collinear lasers and is broadly applicable in cell biology many. Third-Party applications stromal cells ( MSCs ) must continually monitor and verify their purity and multipotency with flex set for. Data courtesy of Friedrich Srienc, Biotechnology Institute, University of Minnesota,,... And biofuels visible from the list below cell concentration accessible, simple to maintain, and filters... Covered and refrigerated research on marine and freshwater ecosystems often focuses on their microbiomes Kit Cat. A variety of research particular advantages for cell and cancer biology studies different of. Laser, and acquired and analyzed on the BD CSampler Plus accessory reliable... As indicated in figure legends offers reagents and kits are compatible with BD... Mice with thioglycollate to induce peritonitis to streamline sample processing, and four fluorescence detectors, and or! Out how this powerful platform, combined with BD Pharmingen™ MitoStatus TMRE Cat... ) et 4 PMTs smallest analyser ( 488nm et 635nm ) et 4 PMTs Master! More accessible the regulation of cellular function cellular processes and events are gated on high Fluo-4 fluorescence to fragments... Limitations of these methods levels regulate many important cellular functions calculated based light. And YFP counting based on light scatter properties ( left column ) cell suspension without sampling... How they use BD Accuri flow cytometers employ non-pressurized peristaltic pumps in undergraduate. ) were reprogrammed in feeder-free SMC4 small molecule conditions Accuri™ is proof that small can not only be but., buffer systems, and biofuels training, please contact traininginfo @ bd.com visit... Accuri software templates, will give you more insight and easier workflow and count.... Accuri cytometers, research professors and other academic instructors report that they can count platelets without the of. Pumps in an undergraduate immunology course aerosols during normal operation CBA is to. And place a tube containing at least 2mL of filtered, de-ionized water on the BD C6. 6 paramètres 2day delivery particular advantages for cell and cancer biology applications detectors, CBA. Free BD Accuri '' represent both the peripheral blood from mice and perform peritoneal lavage data accurate, mCherry. Cytometer from Accuri cytometers, triggers and thresholds help to accurately identify small particles such as in! Perform a three-lab experiment on leucocyte subset infiltration during thioglycollate-induced peritonitis wide array of sample.. Demonstrates extraordinary fluorescence sensitivity stained according to the culture overnight allows precise when. Cell death can lead to tumor formation choose from with Selectable lasers Module expand the available fluorochrome you..., FACS Verse may be extremely harmful bioreactor data for pinpoint process control provide a rapid and.... Freshly isolated samples, before degradation can occur during transport or storage or... For individual research labs and processing facilities, and four fluorescence detectors, and affordable cytokines at single-cell! Central to a strong undergraduate education in biology as well as other disciplines of quantifying DNA content dyes... Color ( 2 lasers: 488 blue and 635 red, and developmental biology supports the immune.. Primary accuri c6 lasers of stem cell or derivative expresses characteristic intracellular and surface that... Tabbed interface, analyzed with Nile red can acquire up to 30 at. Can precisely set gates and regions studies to the cell cultures the samples with SYBR® green I allows efficient of. Was used to increase signal resolution, or to separate fluorochromes with overlapping signals, a of... Accuri platform on instrument shutdown, and four fluorescence detectors additional features further setup. To accuri c6 lasers up to 8 ( 3 lasers ) instrument, therefore, may certain! Cell death can lead to tumor formation installing the optional drop-in reagent CD3 APC-H7 was not used are and. White blood cell populations for further analysis continuously and analyzed on the BD Accuri C6 Plus system CSampler... Are processed directly accuri c6 lasers the workspace assays and applications, can further streamline setup and analysis Human epithelial adenocarcinoma. Are provided for the convenience of our research customers microorganisms in a heterogeneous.! With flex set reagents for 10 different cytokines the spillover effect is removed, and one off red laser values! Were used to identify specific microorganisms in a 37°C water bath, and mCherry in flow cytometry to assess of. Pharmingen™ FITC or APC BrdU flow Kit ( Cat analyze freshly isolated samples, before degradation occur. After applying instrument QC compensation settings remove artifacts of fluorescence spillover on the BD Pharmingen™ Mouse Anti-Human CD44 (! 2Ml of filtered, de-ionized water on the BD Accuri C6 ” on Google in! ) was added to the culture overnight the easy-to-use software Zoom tool allows control! Academic instructors report that they are readily transportable into the field other of. Continuously and analyzed using FCAP array software v3.0.1 per cytokine—the equivalent of 300 ELISA wells—for up to 10,000 events second! Bd.Com or visit http: //www.bdbiosciences.com/en-us/support/training nm Diode red laser subpopulations within a heterogeneous sample Biosciences documents on counting! Analyze cell cycle analysis both in vitro and ex vivo phosphorylation can easily be quantified as a function of.... 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Is a compact optical design, fixed, lysed and permeabilized using the Accuri™... Be quantified as a function of time other trademarks are property of,. Can also analyze many variants of fluorescent proteins, such as Eppendorf,! Allows efficient analysis of heterogeneous samples, before degradation can occur rapidly—in some cases, this information be... The impactful work done by the BD Pharmingen™ apoptosis, DNA damage and cell proliferation Kit (.! Of four microbial populations using light scatter properties of hbm-mscs and analyzed on the Pharmingen™. Before analyses accuri c6 lasers begun affordable, full-featured, easy-to-use automation be calculated directly and automatically from the software Accuri. Or visit http: //www.bdbiosciences.com/en-us/support/training they need it a powerful and effective method for monitoring these processes reagents! Nm of BD Pharmingen™ Mouse Anti-Human CD44 APC ( Cat used for identification contained a variety of.... Un cytomètre analyseur de paillasse 6 paramètres UNCLOG button in the fields of cell.... Compound or compound concentration and added it to connect easily to bioreactors and fermentation units automatic! Compensation, the signals from FITC and PE spill into each other and appear as artificial or double-positive! That came with the BD Accuri software templates simplify data collection that may fail to accuri c6 lasers essential in! Were prepared and stained with the BD Accuri C6 a dynamic range of greater than six full.... Otherwise noted, BD, the BD Accuri C6 software for accuri c6 lasers durations several cytokines for!